Fundamentals of Enzyme Kinetics by Athel Cornish-Bowden

By Athel Cornish-Bowden

Now in its fourth version, this textbook is among the few titles world wide to hide enzyme kinetics in its complete scope and the single one to incorporate its implications for bioinformatics and structures biology. Multi-enzyme complexes and cooperativity are consequently handled in additional aspect than in the other textbook out there.
The revered and renowned writer is without doubt one of the such a lot skilled researchers into the subject and writes with amazing type and didactic readability. As with the former variants, he offers right here steady-state kinetics and quickly reactions, supplementing every one bankruptcy with difficulties and strategies. For the 1st time, this variation includes a better half web site delivering all figures in color
www.wiley-vch.de/home/fundenzykinet

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Fundamentals of Enzyme Kinetics

Now in its fourth variation, this textbook is among the few titles around the world to hide enzyme kinetics in its whole scope and the single one to incorporate its implications for bioinformatics and structures biology. Multi-enzyme complexes and cooperativity are as a result taken care of in additional element than in the other textbook out there.

Extra info for Fundamentals of Enzyme Kinetics

Example text

Integrated Michaelis-Menten equation 35 If we write the Michaelis-Menten equation in its usual form as ν = dp/dt = Vs/(Km + s) (cf. 7), it is an equation in three variables, p , t and s. As such it cannot be integrated directly, but one of the three variables can readily be removed by means of the stoicheiometric relationship s + ρ = s0. g. e. a = —Km In s0 . 17) where ρ has been written as s 0 — s and V and Km have been replaced a by apparent values F P P and respectively. 16), and a in these cases F P P and A^fPP are not equal to V and Km .

A plot of ρ against t) is essentially straight for an appreciable period, and in such cases estimation o f the initial slope, and hence the initial rate, is subjective and liable to be biased. Much of this subjectivity and bias can be removed by using an integrated form o f the rate equation, as I shall now describe. Integrated Michaelis-Menten equation 35 If we write the Michaelis-Menten equation in its usual form as ν = dp/dt = Vs/(Km + s) (cf. 7), it is an equation in three variables, p , t and s.

If A and Ρ are released from their respective complexes with the same rate constant), then both expressions have common factors o f (k_2 + k+2 + k+3 ) in numerators and denominators, so Product inhibition 33 ? Κ* = k_x /k+x = Κ* and K m = k+3/k_3 = K* (Cornish-Bowden, 1976). 15 that A ? K K = 0 and so _ ρ ^ = κ m where Κ is the equilibrium constant o f the reaction. This is an important result, and is known as the Haldane relationship (Haldane, 1930). 15, not merely for the simple two-step Michaelis-Menten mechanism.

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