By S.F.Y. Li
Capillary Electrophoresis (CE) has had a really major impression at the box of analytical chemistry lately because the strategy is able to very excessive answer separations, requiring purely small quantities of samples and reagents. moreover, it may be easily tailored to automated pattern dealing with and genuine time information processing. Many new methodologies according to CE were mentioned. quick, reproducible separations of super small quantities of chemical substances and biochemicals, together with peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, supplementations, inorganic ions, prescribed drugs and environmental pollution were established. quite a lot of functions were constructed in tremendously varied fields, akin to chemical, biotechnological, environmental and pharmaceutical research.
This booklet covers all facets of CE, from the foundations and technical features to an important purposes. it's meant to satisfy the turning out to be desire for an intensive and balanced remedy of CE. The booklet will function a accomplished reference paintings. either the skilled analyst and the newcomer will locate the textual content beneficial.
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Extra resources for Capillary electrophoresis: principles, practice, and applications
For electrophoretic separation of both large and small molecules. CGE with fraction collection has also been performed [41,50] for micropreparative purification of macromolecules. Karger and co-workers [49,50] achieved extremely high separation efficiency (up to 30 million theoretical plates per meter) using gel-filled capillary columns. The capillaries were filled with polyacrylamide gels which contained sodium dodecyl sulfate. This technique is referred to as capillary SDS-PAGE separation and has been used for the separation of proteins, polynucleotides and DNA fragments [48SO].
For fraction collection, commercially available fraction collectors can be utilized. In CE, the column diameter is limited by the efficiency of heat dissipation. Since the heat gradient between the center of the capillary and the walls is proportional to the square of the radius, smaller capillaries enhance heat dissipation. So far, capillaries of 2-200 p m diameter and 10-100 cm length are most commonly used in CE separations. The use of multiple capillaries permits larger sample capacity in C E [100,lO11.
Otsuka and S. Terabe, J. Microcol. , 1 (1989) 150 T. Tsuda, K Normura and G. Nakagawa, J. Chromatogr. J. O. Cole, Anal. T. J. Sepaniak, Anal. , 59 (1988) 1466 M. Martin, G. Guiochon, Y.