Bioreactor Systems for Tissue Engineering II: Strategies for by Susanne Wolbank, Martijn van Griensven (auth.), Cornelia

By Susanne Wolbank, Martijn van Griensven (auth.), Cornelia Kasper, Martijn van Griensven, Ralf Pörtner (eds.)

Alternative resources of grownup Stem Cells: Human Amniotic Membrane, by means of S. Wolbank, M. van Griensven, R. Grillari-Voglauer, and A. Peterbauer-Scherb;
Mesenchymal Stromal Cells Derived from Human Umbilical twine Tissues: Primitive Cells with power for medical and Tissue Engineering functions, via P. Moretti, T. Hatlapatka, D. Marten, A. Lavrentieva, I. Majore, R. Hass and C. Kasper;
Isolation, Characterization, Differentiation, and alertness of Adipose-Derived Stem Cells, by means of J. W. Kuhbier, B. Weyand, C. Radtke, P. M. Vogt, C. Kasper and okay. Reimers;
Induced Pluripotent Stem Cells: features and views, through T. Cantz and U. Martin;
Induced Pluripotent Stem phone expertise in Regenerative medication and Biology, by way of D. Pei, J. Xu, Q. Zhuang, H.-F. Tse and M. A. Esteban;
Production approach for Stem mobile dependent healing Implants: enlargement of the construction cellphone Line and Cultivation of Encapsulated Cells, by way of C. Weber, S. Pohl, R. Poertner, P. Pino-Grace, D. Freimark, C. Wallrapp, P. Geigle and P. Czermak;
Cartilage Engineering from Mesenchymal Stem Cells, through C. Goepfert, A. Slobodianski, A.F. Schilling, P. Adamietz and R. Poertner;
Outgrowth Endothelial Cells: resources, features and strength purposes in Tissue Engineering and Regenerative medication, by way of S. Fuchs, E. Dohle, M. Kolbe, C. J. Kirkpatrick;
Basic technology and medical software of Stem Cells in Veterinary drugs, by way of I. Ribitsch, J. Burk, U. Delling, C. Geißler, C. Gittel, H. Jülke, W. Brehm;
Bone Marrow Stem Cells in medical software: Harnessing Paracrine Roles and area of interest Mechanisms, through R. M. El Backly, R. Cancedda;
Clinical program of Stem Cells within the Cardiovascular approach, C. Stamm, okay. Klose, Y.-H. Choi;

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Additional info for Bioreactor Systems for Tissue Engineering II: Strategies for the Expansion and Directed Differentiation of Stem Cells

Example text

In contrast to other progenitor cell populations, such as, for instance, hematopoeitic stem cells, there is currently no specific marker available defining human MSCs. The expression of a set of markers combined with the demonstration of in vitro multi-lineage differentiation potential is necessary to identify MSCs in UCderived cell populations. Table 1 summarizes extracellular and intracellular molecules expressed by UC-MSCs reported in the literature up to July 2009. Fig. 7 CFU-F assay of UC-derived stromal cells 38 P.

Because there is currently no consensus set of markers allowing the identification of MSCs and considering the fact the definition criteria for stem cell is not unanimously accepted [39], it appeared unwise to apply the term “stem cell” for mesenchymal cell population. In this context, ISCT proposed to term plasticadherent fibroblast cultures “multipotent mesenchymal stromal cells” (MSC) [40] and published in 2006 the minimal criteria defining these cells [41]. UC-derived stromal cells meet the basic criteria defined by the ISCT, namely the adherence to plastic, the expression of a set of specific surface antigens (see below), and a multipotent differentiation potential (discussed in paragraph 5 Differentiation Potential).

48 Abbreviations ALP b-FGF BM BrdU CCE CD CFSE CFU-F DAPI ESC GMP GvHD HA HLA HUCPVC ISCT MLC MSC MTT PBL PBMC PBS UC UCB UCMS VEGF WJ WJC Alkaline phosphatase Basic fibroblast growth factor Bone marrow 5-Bromo-2-deoxyuridine Counterflow centrifugal elutriation Cluster of differentiation Carboxyfluorescein diacetate succinimidyl ester Colony forming unit-fibroblast 40 ,6-Diamidino-2-phenylindole Embryonic stem cell Good manufacturing practice Graft-versus-host disease Hyaluronic acid Human leukozyte antigen Human umbilical cord perivascular cells International Society for Cellular Therapy Mixed lymphocyte culture Mesenchymal stromal cell 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide Peripheral blood lymphocytes Peripheral blood mononuclear cell Phosphate buffered saline Umbilical cord Umbilical cord blood Umbilical cord matrix cells Vascular endothelial growth factor Wharton’s jelly Wharton’s jelly cell Mesenchymal Stromal Cells Derived from Human Umbilical Cord Tissues 31 1 Introduction The engineering of stem cells to restore defect functions in human tissues is an exciting challenge in the field of regenerative medicine.

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