A Guide to Protein Isolation by C. Dennison

By C. Dennison

This article takes the reader on a guided travel in the course of the philosophical and actual foundations of protein isolation. aimed toward a scholar readership, it is going to even be very valuable to lifestyles technological know-how researchers confronted with the duty of keeping apart a protein for the 1st time. The good judgment of the general method of separating a protein is defined and the actual ideas of every separation technique are made transparent by way of easy types and analogies, drawn from daily studies. The author's objective has been to deepen the readers' perception into protein isolation tools, in order that they might take on new difficulties and maybe devise new techniques to outdated difficulties. the various equipment defined are drawn from the author's personal study and are therefore uniquely defined right here: examples are three-phase partitioning, non-linear electrophoresis, and an easy method of buffer making.

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Biol. Chem. 73, 627-650. 12. Peterson, G. L. (1979) Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall. Anal. Biochem. 100, 201-220. 13. Smith, P. , Krohn, R. , Hemianson, G. , Mallia, A. , Gartner, F. , Provenzano, M. , Fugimoto, E. , Goeke, N. , Olsen, B. J. and Klenk, D. C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85. 14. Wiechelman, K. , Braun, R. D. and Fitzpatrick, J. D. (1988) Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation.

What is “vapour pressure”? Consider the set-up shown in Fig. e. with no other gases present, and with a pressure gauge to monitor the (vapour) pressure. In this way, the vapour pressure can be measured as the temperature of the set-up is changed. If this is done, it will be noticed that the measured pressure (the vapour pressure) changes with the temperature as shown in Fig. 25. e. the total pressure will be the vapour pressure at that temperature plus the (partial) pressures of the other gases.

Itzhaki, R. F. and Gill, D. M. (1 964) A micro-biuret method for estimating protein. Anal. Biochem. 9, 401-410. 10. Lowry, O. , Rosebrough, N. , Farr, A. L. and Randall, R. J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275. 1 1 Folin, O. and Ciocalteu, V. (1927) Tyrosine and tryptophan determination in proteins. J. Biol. Chem. 73, 627-650. 12. Peterson, G. L. (1979) Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.

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